Salubrious Effect of Rottlerin on Hyperoxaluria Induced Oxidative Damage in Rats

Objective: To investigate the in vitro oxidant scavenging properties of rottlerin and to study the potential role of rottlerin on ethylene glycol induced nephrocalcinosis in rats. Methods: In vitro oxidant scavenging properties of rottlerin were studied along with its effect on in vitro calcium phosphate mineralization. For the in vivo studies, hyperoxaluria was induced by administering 0.4 % ethylene glycol and 1 % ammonium chloride in drinking water to male wistar rats for 9 d. Rottlerin was administered intraperitoneally at 1mg/kg/d along with the hyperoxaluric agent. Total thiols content, activities of glutathione-S-transferase (GST), glutathione reductase (GR), Citrate synthase (CS), isocitrate dehydrogenase (ICDH), ATPase and urinary parameters were studied. Results: Rottlerin showed in vitro DPPH, superoxide, and ABTS radical scavenging activity along with inhibition of calcium phosphate mineralization in an in vitro homogeneous system. The diminished activities of GST, GR, ICDH, CS, ATPase and level of total thiols were considerably stabilized by rottlerin, suggesting that rottlerin provides protection against oxalate induced oxidative damage. Conclusion: We suggest that rottlerin protects the integrity of the renal cell by stabilizing the free-radical mediated damage. Thus, the present study reveals that the antioxidant nature of rottlerin protects the renal cells against oxalate-induced injury and thus, rottlerin may prevent against hyperoxaluria induced oxidative damage.